Impurity Library

Peptide Impurity Reference Standards

Browse standards for synthesis and degradation-related impurities of semaglutide, tirzepatide and retatrutide.

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Reference standard catalog preview
Name	Category
[D-His1]SemaglutideSemaglutide	D-Epimers
[β-Asp9]TirzepatideTirzepatide	Linkage isomers
[Des-Tyr1]RetatrutideRetatrutide	Deletion sequences

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01 / Library overview

342 standards across five impurity classes

106

D-Epimers

Single D-amino-acid substitutions at each sequence position — epimerization markers of synthesis and storage.

124

Deletion sequences

Des-amino-acid forms missing one residue — the classic failure sequences of SPPS.

Insertion / double residues

Double-coupled residues from incomplete capping during synthesis.

Linkage isomers

β-Asp, γ-Glu and α-Glu side-chain linkage variants from aspartimide rearrangement and mis-coupling.

Side-chain & other

Acetylated, substituted and C-terminus-modified variants.

02 / Search the library

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101 standards

Name	Sequence	Data
[D-His1]Semaglutide	Sequence H-D-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[D-Glu3]Semaglutide	Sequence H-His1-Aib2-D-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[D-Thr5]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-D-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[D-Allo-Thr5]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-(D-Allo-Thr5)-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[D-Phe6]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-D-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[D-Allo-Thr7]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-(D-Allo-Thr7)-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[D-Thr7]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-D-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[D-Ser8]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-D-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[D-Asp9]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-D-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[β-Asp9]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-β-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[D-β-Asp9]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-D-β-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[D-Val10]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-D-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[D-Ser11]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-D-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[D-Ser12]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-D-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[D-Tyr13]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-D-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[D-Leu14]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-D-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[D-Glu15]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-D-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[D-Gln17]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-D-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[D-Ala18]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-D-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[D-Ala19]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-D-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[D-Lys20]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-D-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[D-Glu21]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-D-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[D-Phe22]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-D-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[D-Ile23]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-D-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[D-Allo-Ile23]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-(D-Allo-Ile23)-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[D-Ala24]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-D-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[D-Trp25]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-D-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[D-Leu26]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-D-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[D-Val27]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-D-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[D-28Arg]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-D-Arg28-Gly29-Arg30-Gly31-0H	MS · HPLC
[D-30Arg]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-D-Arg30-Gly31-0H	MS · HPLC
[α-Glu](Side Chain)-Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[D-γGlu](Side Chain)-Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-(D-γ-Glu)-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Des-His1]Semaglutide	Sequence Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Des-His1-Aib2]Semaglutide	Sequence H-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Des-Aib2]Semaglutide	Sequence H-His1-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Des-Glu3-Gly4]Semaglutide	Sequence H-His1-Aib2-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Des Glu3]Semaglutide	Sequence H-His1-Aib2-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-0H	MS · HPLC
[Des-Gly4]Semaglutide	Sequence H-His1-Aib2-Glu3-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Des Thr5]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Des Phe6]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Des Thr7]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Des Ser8]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Des Asp9]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Des Val10]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Des Ser11]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Des Ser12]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Des Tyr13]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Des Leu14]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Des Glu15]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Des Gly16]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Des Gln17]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Des Ala18]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Des Ala19]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Des Side Chain]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Des Lys20(Side Chain)]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Des Glu21]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Des Phe22]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Des Ile23]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Des Ala24]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Des Trp25]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Des Leu26]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Des Val27]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Des Arg28]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Gly29-Arg30-Gly31-OH	MS · HPLC
[Des (Arg28 Gly31]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Gly29-Arg30-OH	MS · HPLC
[Des Gly29]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Arg30-Gly31-OH	MS · HPLC
[Des Arg30]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Gly31-OH	MS · HPLC
[Des (Arg30 Gly31)]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-OH	MS · HPLC
[Des Gly31]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-OH	MS · HPLC
Des [AEEA] (Side chain) Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Des γE]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Des Oct]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Double His1]Semaglutide	Sequence H-His1-His-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Double Glu3]Semaglutide	Sequence H-His1-Aib2-Glu3-Glu-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Double Gly4]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Gly-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Double Thr5]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Thr-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Double Phe6]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Phe-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Double Thr7]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Thr-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Double Ser8]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Ser-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Double Asp9]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Asp-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Double Val10]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Val-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Double Ser11]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Double Tyr13]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Tyr-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Double Leu14]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Leu-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Double Glu15]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Glu-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Double Gly16]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gly-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Double Gln17]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Gln-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Double Ala18]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Double Glu21]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Glu-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Double Phe22]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Phe-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Double Ile23]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ile-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Double Ala24]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Ala-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Double Trp25]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Trp-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Double Leu26]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Leu-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Double Val27]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Val-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Double Gly29]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Gly-Arg30-Gly31-OH	MS · HPLC
[Double Arg30]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Arg-Gly31-OH	MS · HPLC
[Double Gly31]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-Gly-OH	MS · HPLC
[Double AEEA]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
[Glu17]Semaglutide	Sequence H-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Glu17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC
Acetylated-Semaglutide	Sequence Ac-His1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Val10-Ser11-Ser12-Tyr13-Leu14-Glu15-Gly16-Gln17-Ala18-Ala19-Lys20(AEEA-AEEA-γ-Glu-Oct)-Glu21-Phe22-Ile23-Ala24-Trp25-Leu26-Val27-Arg28-Gly29-Arg30-Gly31-OH	MS · HPLC

123 standards

Name	Sequence	Data
[D-Tyr1]Tirzepatide	Sequence H-D-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Glu3]Tirzepatide	Sequence H-Tyr1-Aib2-D-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Thr5]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-D-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Phe6]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-D-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Thr7]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-D-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Ser8]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-D-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Asp9]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-D-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[β-Asp9]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-β-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-β-Asp9]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-D-β-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Tyr10]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-D-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Ser11]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-D-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Ile12]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-D-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Leu14]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-D-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[β-Asp15]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-β-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-β-Asp15]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-D-β-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Asp15]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-D-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Lys16]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-D-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Ile17]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-D-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Ala18]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-D-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Gln19]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-D-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Lys20]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-D-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Ala21]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-D-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Phe22]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-D-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Val23]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-D-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Gln24]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-D-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Trp25]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-D-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Leu26]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-D-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Ile27]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-D-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Ala28]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-D-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Pro31]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-D-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Ser32]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-D-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Ser33]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-D-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Ala35]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-D-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Pro36]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-D-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Pro37]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-D-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Pro38]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-D-Pro38-Ser39-NH2	MS · HPLC
[D-Ser39]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-D-Ser39-NH2	MS · HPLC
[D-γGlu](Side Chain)-Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-D-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des Tyr1~Gly4]Tirzepatide	Sequence H-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des Tyr1]Tirzepatide	Sequence H-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des Aib2]Tirzepatide	Sequence H-Tyr1-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des- Tyr1-Aib2]-Tirzepatide	Sequence H-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des Glu3]Tirzepatide	Sequence H-Tyr1-Aib2-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des Gly4]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des- Glu3-Gly4]Tirzepatide	Sequence H-Tyr1-Aib2-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des Thr5]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des Phe6]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des Thr7]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des Ser8]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des Asp9]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des Tyr10]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des Ser11]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des Ile12]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des Aib13]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des- Ile12-Aib13]	Sequence Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des Leu14]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des Asp15]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des Lys16]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des Ile17]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des Ala18]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des- Ile17-Ala18]-Tirzepatide	Sequence Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des Gln19]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des Side Chain]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des Lys20(Side Chain)]-Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des AEEA(Side Chain)]-Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des γ-Glu(Side Chain)]-Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des Ala21]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des Phe22]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des Val23]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des Gln24]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des Trp25]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des Leu26]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des Ile27]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des Ala28]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des Gly29]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des Pro31]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des Ile27-Ala28]-Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des Gly29-Gly30]-Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des Ser32]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des Gly34]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des Ala35]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des Pro36]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des-Pro36-Pro37]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des Ser39]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-NH2	MS · HPLC
[Double Tyr1]Tirzepatide	Sequence H-Tyr1-Tyr-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double Glu3]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Glu-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double Gly4]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Gly-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double Thr5]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Thr-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double Phe6]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Phe-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double Thr7]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Thr-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double Ser8]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Ser-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double Asp9]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Asp-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double Tyr10]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Tyr-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double Ser11]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ser-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double -Ile12]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12--Ile-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double Leu14]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Leu-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double Asp15]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Asp-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double Lys16]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Lys-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double Ile17]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ile-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double Ala18]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Ala-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double Gln19]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Gln-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double AEEA]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double Ala21]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Ala-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double Phe22]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Phe-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double Val23]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Val-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double Gln24]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Gln-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double Trp25]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Trp-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double Leu26]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Leu-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double Ile27]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ile-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double Ala28]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Ala-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double Gly29]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double Pro31]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Pro-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double Ser32]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double Gly34]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Gly-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double Ala35]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Ala-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double Pro36]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double Ser39]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-Ser-NH2	MS · HPLC
[Acetylated]Tirzepatide	Sequence Ac-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Nva12]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Nva12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Glu19]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Glu19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Glu24]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Glu24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Ser39-OH]Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-γ-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-OH	MS · HPLC
[α-Glu](Side Chain)Tirzepatide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Aib13-Leu14-Asp15-Lys16-Ile17-Ala18-Gln19-Lys20(AEEA-AEEA-Glu-C20-diacid)-Ala21-Phe22-Val23-Gln24-Trp25-Leu26-Ile27-Ala28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC

118 standards

Name	Sequence	Data
[D-Tyr1]Retatrutide	Sequence H-D-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Gln3]Retatrutide	Sequence H-Tyr1-Aib2-D-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Thr5]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-D-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Phe6]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-D-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Thr7]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-D-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Ser8]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-D-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Asp9]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-D-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Tyr10]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-D-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Ser11]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-D-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Ile12]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-D-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-(α)MeLeu13]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-D-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Leu14]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-D-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Asp15]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-D-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Lys16]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-D-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Lys17]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-D-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Ala18]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-D-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Gln19]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-D-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Ala21]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-D-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Phe22]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-D-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Ile23]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-D-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Glu24]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-D-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Tyr25]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-D-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Leu26]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-D-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Leu27]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-D-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Glu28]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-D-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Pro31]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-D-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Ser32]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-D-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Ser33]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-D-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Ala35]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-D-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Pro36]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-D-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Pro37]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-D-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Pro38]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-D-Pro38-Ser39-NH2	MS · HPLC
[D-Ser39]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-D-Ser39-NH2	MS · HPLC
[D-β-Asp9]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-D-β-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[β-Asp9]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-β-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-β-Asp15]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-D-β-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[β-Asp15]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-β-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
Nε17-[D-γ-Glu2]-Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-D-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[γ-Glu24]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-γ-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[γ-Glu28]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-γ-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[α-Glu](Side Chain)Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-α-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Allo-Thr5]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-D-Allo-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Allo-Thr7]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-D-Allo-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Allo-Ile12]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-D-Allo-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[D-Allo-Ile23]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22--D-Allo-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des-Tyr1]Retatrutide	Sequence H-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des-Aib2]Retatrutide	Sequence H-Tyr1-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des-Gln3]Retatrutide	Sequence H-Tyr1-Aib2-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des-Gly4]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des-Thr5]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des-Phe6]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des-Thr7]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des-Ser8]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des-Asp9]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des-Tyr10]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des-Ser11]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des-Ile12]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des-(α)MeLeu13]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des-Leu14]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des-Asp15]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des-Lys16]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des Side Chain]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des-Lys17(Side Chain)]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des-Eicosanedioicacid]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des-γ-Glu]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des-AEEA]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des-Ala18]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des-Gln19]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des-Aib20]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des-Ala21]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des-Phe22]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des-Ile23]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des-Glu24]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des-Tyr25]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des-Leu26]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des-Glu28]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des-Gly29]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des-Pro31]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des-Ser32]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des-Gly34]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des-Ala35]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des-Pro36]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Des-Ser39]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-NH2	MS · HPLC
[Des-Gly29-Gly30]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double Tyr1]Retatrutide	Sequence H-Tyr1-Tyr-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double-Gln3]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gln-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double-Gly4]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Gly-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double-Thr5]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Thr-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double-Phe6]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Phe-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double-Thr7]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Thr-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double-Ser8]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Ser-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double-Asp9]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Asp-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double-Tyr10]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Tyr-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double-Ser11]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ser-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double-Ile12]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-Ile-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double-Leu14]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Leu-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double-Asp15]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Asp-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double-Lys16]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double-AEEA]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double-Ala18]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Ala-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double-Gln19]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Gln-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double-Ala21]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Ala-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double-Phe22]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Phe-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double-Ile23]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Ile-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double-Glu24]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Glu-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double-Tyr25]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Tyr-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double-Leu26]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double-Glu28]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Glu-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double-Gly29]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double-Pro31]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Pro-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double-Ser32]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double-Gly34]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Gly-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double-Ala35]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Ala-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double-Pro36]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Double-Ser39]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-Ser-NH2	MS · HPLC
[Ser39-OH]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Glu3]Retatrutide	Sequence H-Tyr1-Aib2-Glu3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Gln19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC
[Glu19]Retatrutide	Sequence H-Tyr1-Aib2-Gln3-Gly4-Thr5-Phe6-Thr7-Ser8-Asp9-Tyr10-Ser11-Ile12-(α)MeLeu13-Leu14-Asp15-Lys16-Lys17(Eicosanedioicacid-γ-Glu-AEEA)-Ala18-Glu19-Aib20-Ala21-Phe22-Ile23-Glu24-Tyr25-Leu26-Leu27-Glu28-Gly29-Gly30-Pro31-Ser32-Ser33-Gly34-Ala35-Pro36-Pro37-Pro38-Ser39-NH2	MS · HPLC

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Characterization guides by molecule:Semaglutide →Tirzepatide →Retatrutide →

Research and regulatory context

On July 28, 2026, FDA withdrew its 2021 guidance on ANDAs for certain highly purified synthetic peptide drug products and published revised draft product-specific guidances for certain peptide products. Check the applicable documents and validation scope for the product, dosage form, market and intended study.

FDA · 2026-07-28 ICH Q2(R2)

Impurity standards · custom synthesis

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