SV-AUC peptide aggregation and self-association
Sedimentation velocity analytical ultracentrifugation records how components sediment in free solution. The sedimentation coefficient distribution can help compare monomer, oligomer and soluble aggregate populations under defined conditions.
Request a technical assessmentResearch questions
- Does the high-molecular-weight population observed by SEC persist under independently defined solution conditions?
- How does the sedimentation profile change across concentrations of a self-associating peptide such as semaglutide?
- Do formulations, batches or pre- and post-change samples show differences in their sedimentation profiles?
What the report can include
- Sedimentation coefficient distributions c(s) and overlays across samples or test conditions.
- Relative signal contributions of resolved populations, including integration ranges and assumptions.
- Where supported, standardized sedimentation coefficients s20,w or model-based estimates of molar mass.
How to interpret the results
Sedimentation depends on mass, shape and solvent conditions. Assigning oligomeric states or quantifying low-level species requires adequate resolution and signal. Insoluble material and species outside the measurement window may require additional methods. Compare SV-AUC and SEC with the effects of dilution, column interactions and sample handling in mind.
Before sending samples
Provide the molecule, sample form, concentration, formulation, batch count and storage conditions. For SEC follow-up, describe the peak of interest, the SEC conditions and the comparison you need to make.